1ORY
FLAGELLAR EXPORT CHAPERONE IN COMPLEX WITH ITS COGNATE BINDING PARTNER
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-BM |
| Synchrotron site | APS |
| Beamline | 17-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-11-10 |
| Detector | MARRESEARCH |
| Spacegroup name | I 41 3 2 |
| Unit cell lengths | 166.241, 166.241, 166.241 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 100.000 * - 2.500* |
| R-factor | 0.21694 |
| Rwork | 0.215 |
| R-free | 0.24700 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1oqp |
| RMSD bond length | 0.060 * |
| RMSD bond angle | 2.100 * |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.19) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 * | 2.550 |
| High resolution limit [Å] | 2.500 * | 2.500 * |
| Rmerge | 0.044 * | 0.367 * |
| Number of reflections | 13539 * | 1492 * |
| <I/σ(I)> | 15.66 | 1.99 |
| Completeness [%] | 99.4 * | 99.9 |
| Redundancy | 4.4 * | 4.8 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 * | 293 | 10% isopropanol, 200mM Lithium sulfate, citrate-phosphate buffer, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | MES | 20 (mM) | pH6.5 |
| 3 | 1 | drop | 150 (mM) | ||
| 4 | 1 | drop | dithiothreitol | 2 (mM) | |
| 5 | 1 | reservoir | ammonium sulfate | 2.0 (M) | |
| 6 | 1 | reservoir | MES | 100 (mM) | pH5.4-6.2., or cacodylate |
