1ORU
Crystal Structure of APC1665, YUAD protein from Bacillus subtilis
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-02-09 |
| Detector | SBC-3 |
| Wavelength(s) | 0.97943, 0.97962, 0.961 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 42.652, 87.202, 122.249 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.070 - 1.800 |
| R-factor | 0.198 |
| Rwork | 0.196 |
| R-free | 0.22500 |
| Structure solution method | MAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.300 |
| Data reduction software | d*TREK |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.600 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.091 | 0.522 |
| Number of reflections | 43357 | |
| Completeness [%] | 99.6 | 98 |
| Redundancy | 8.7 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | HEPES, PEG 400, Ammonium Sulfate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
