1ORT
ORNITHINE TRANSCARBAMOYLASE FROM PSEUDOMONAS AERUGINOSA
Experimental procedure
| Temperature [K] | 294 |
| Detector technology | DIFFRACTOMETER |
| Collection date | 1993-01-20 |
| Detector | ENRAF-NONIUS FAST |
| Spacegroup name | P 1 |
| Unit cell lengths | 110.360, 126.420, 134.540 |
| Unit cell angles | 85.07, 59.24, 111.97 |
Refinement procedure
| Resolution | 5.500 - 3.000 |
| R-factor | 0.216 |
| Rwork | 0.216 |
| RMSD bond length | 0.015 * |
| RMSD bond angle | 4.010 * |
| Data reduction software | MADNES |
| Phasing software | X-PLOR (3.0) |
| Refinement software | X-PLOR (3.0) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 3.000 |
| Rmerge | 0.050 |
| Total number of observations | 116334 * |
| Number of reflections | 92630 |
| Completeness [%] | 85.0 |
| Redundancy | 1.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7 | 21 * | pH 7.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 24 (mg/ml) | |
| 2 | 1 | reservoir | HEPES | 50 (mM) | |
| 3 | 1 | reservoir | ammonium sulfate | 50 (%(w/v)) | |
| 4 | 1 | reservoir | dithiothreitol | 1 (mM) | |
| 5 | 1 | reservoir | EDTA | 1 (mM) | |
| 6 | 1 | drop | HEPES | 50 (mM) |
