1ORP
Structure of a Trapped Endonuclease III-DNA Covalent Intermediate: Estranged-Adenine Complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE A1 |
Synchrotron site | CHESS |
Beamline | A1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-01-19 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.9363 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 67.732, 106.516, 41.661 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 * - 2.200 |
R-factor | 0.241 |
Rwork | 0.241 |
R-free | 0.26200 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 1orn |
RMSD bond length | 0.008 |
RMSD bond angle | 19.100 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.280 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.056 | 0.341 |
Number of reflections | 15614 | |
<I/σ(I)> | 24 | 4.4 |
Completeness [%] | 97.9 | 99.2 |
Redundancy | 5.1 | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | PEG 8000, Tris, beta-mercaptoethanol , pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | PEG 8000 | ||
2 | 1 | 1 | Tris | ||
3 | 1 | 1 | beta-mercaptoethanol | ||
4 | 1 | 2 | PEG 8000 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | Tris | 100 (mM) | pH8.5 |
2 | 1 | reservoir | PEG8000 | 20-25 (%(w/v)) | |
3 | 1 | reservoir | 5 (mM) |