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TET REPRESSOR, CLASS D IN COMPLEX WITH 9-(N,N-DIMETHYLGLYCYLAMIDO)-6-DEMETHYL-6-DEOXY-TETRACYCLINE
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X31 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X31 |
| Temperature [K] | 277 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-05 |
| Detector | MARRESEARCH |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 68.940, 68.940, 180.750 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 18.500 - 2.400 |
| R-factor | 0.222 * |
| Rwork | 0.214 |
| R-free | 0.27700 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2tct |
| RMSD bond length | 0.008 * |
| RMSD bond angle | 1.310 * |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 17.200 | 2.470 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.059 * | 0.193 * |
| Number of reflections | 8979 | |
| <I/σ(I)> | 15.6 | 6 |
| Completeness [%] | 96.0 | 98.8 |
| Redundancy | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8 | pH 8.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | 200 (mM) | ||
| 3 | 1 | drop | Tris-HCl | 50 (mM) | |
| 4 | 1 | reservoir | ammonium sulfate | 1 (M) | |
| 5 | 1 | reservoir | 10 (mM) | ||
| 6 | 1 | reservoir | 200 (mM) | ||
| 7 | 1 | reservoir | Tris-HCl | 50 (mM) |
