1ORJ
FLAGELLAR EXPORT CHAPERONE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-08-20 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 87.070, 131.760, 74.898 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 100.000 * - 2.200* |
| R-factor | 0.19163 |
| Rwork | 0.189 |
| R-free | 0.28600 * |
| Structure solution method | MIR |
| RMSD bond length | 0.050 * |
| RMSD bond angle | 1.700 * |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | REFMAC (5.1.19) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 * | 2.350 |
| High resolution limit [Å] | 2.200 * | 2.200 * |
| Rmerge | 0.047 * | 0.285 * |
| Number of reflections | 44213 * | 5127 * |
| <I/σ(I)> | 14.31 | 4.04 |
| Completeness [%] | 94.9 * | 94.3 * |
| Redundancy | 3.25 * | 2.29 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 * | 291 | 2.0M Ammonium sulfate, 100mM MES, 2mM DTT, pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | MES | 20 (mM) | pH6.5 |
| 3 | 1 | drop | 150 (mM) | ||
| 4 | 1 | drop | dithiothreitol | 2 (mM) | |
| 5 | 1 | reservoir | ammonium sulfate | 2.0 (M) | |
| 6 | 1 | reservoir | MES | 100 (mM) | pH5.4-6.2., or cacodylate |
