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CRYSTALLOGRAPHIC STRUCTURE OF A PLP-DEPENDENT ORNITHINE DECARBOXYLASE FROM LACTOBACILLUS 30A TO 3.1 ANGSTROMS RESOLUTION
Experimental procedure
| Spacegroup name | P 6 |
| Unit cell lengths | 195.600, 195.600, 97.600 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | ? - 3.000 |
| R-factor | 0.219 |
| Rwork | 0.219 |
| R-free | 0.26800 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 24.767 * |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 8.000 |
| High resolution limit [Å] | 3.000 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | dialyzing * | 5.8 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG8000 | 7 (%(w/v)) | |
| 2 | 1 | reservoir | potassium phosphate | 50 (mM) | |
| 3 | 1 | reservoir | 100 (mM) | ||
| 4 | 1 | reservoir | EDTA | 0.5 (mM) | |
| 5 | 1 | reservoir | beta-octylglucoside | 0.05 (%) | |
| 6 | 1 | reservoir | 0.02 (%(w/v)) | ||
| 7 | 1 | reservoir | pyridoxal-5'-phosphate | 0.2 (mM) |
