1OQV
Structure of TcpA, the Type IV pilin subunit from the toxin co-regulated pilus of Vibrio cholerae classical biotype
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-11-26 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.975913 |
| Spacegroup name | P 63 |
| Unit cell lengths | 157.317, 157.317, 35.719 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 1.300 |
| R-factor | 0.113 |
| Rwork | 0.116 |
| R-free | 0.17400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1.8 A structure of N-terminally truncated SeMet-labelled TcpA |
| RMSD bond length | 0.010 |
| RMSD bond angle | 2.100 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.370 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.073 | 0.245 |
| Total number of observations | 573481 * | |
| Number of reflections | 115573 | |
| <I/σ(I)> | 5.1 | 1.8 |
| Completeness [%] | 98.4 | 93.9 |
| Redundancy | 4.71 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | PEG 8000, imidazole, sodium chloride, glycerol, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15 (mg/ml) | |
| 2 | 1 | reservoir | PEG8000 | 30 (%) | |
| 3 | 1 | reservoir | imidazole | 0.1 (M) | pH8.0 |
| 4 | 1 | reservoir | 0.2 (M) | pH8.0 | |
| 5 | 1 | reservoir | glycerol | 6 (%) |
