1OQS

Crystal Structure of RV4/RV7 Complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X11
Synchrotron site	EMBL/DESY, Hamburg
Beamline	X11
Spacegroup name	P 1 21 1
Unit cell lengths	74.918, 85.125, 78.159
Unit cell angles	90.00, 95.10, 90.00

Refinement procedure

Resolution	25.000 * - 1.900
R-factor	0.22293
Rwork	0.222
R-free	0.27700 *
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.028 *
RMSD bond angle	2.469 *
Refinement software	REFMAC (5.1.16)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000 *
High resolution limit [Å]	1.900	1.900 *
Rmerge	0.061 *	0.281 *
Number of reflections	74210 *
Completeness [%]	98.1 *	97.9 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	4.5 *	289 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	24 (mg/ml)
2	1	drop	sodium acetate	50 (mM)	pH4.5
3	1	reservoir	MPD	7 (%)
4	1	reservoir	PEG4000	1 (%)
5	1	reservoir		1 (mM)	pH4.5