1OQL
Mistletoe Lectin I from Viscum album complexed with galactose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX7.2 |
| Synchrotron site | SRS |
| Beamline | PX7.2 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-06-12 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.488 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 107.650, 107.650, 311.920 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 * - 3.000 |
| R-factor | 0.201 |
| Rwork | 0.199 |
| R-free | 0.24000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Ricin (PDB 2AAI) |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.720 * |
| Data reduction software | DENZO |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 * |
| High resolution limit [Å] | 2.900 |
| Rmerge | 0.069 |
| Total number of observations | 68126 * |
| Number of reflections | 24175 |
| <I/σ(I)> | 8.8 |
| Completeness [%] | 98.0 |
| Redundancy | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 * | 293 | 0.9M Ammonium sulphate, Glycine buffer, pH 3.4, 0.1M galactose, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 18 (mg/ml) | |
| 2 | 1 | drop | galactose | 0.1 (M) | |
| 3 | 1 | drop | acetate | 0.01 (M) | pH4.0 |
| 4 | 1 | reservoir | ammonium sulfate | 0.9 (M) | |
| 5 | 1 | reservoir | glycine | 0.1 (M) | pH3.4 |
