1OPS

ICE-BINDING SURFACE ON A TYPE III ANTIFREEZE PROTEIN FROM OCEAN POUT

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	277
Detector technology	IMAGE PLATE
Collection date	1993-05
Detector	RIGAKU
Spacegroup name	P 1 21 1
Unit cell lengths	23.060, 40.790, 29.990
Unit cell angles	90.00, 100.60, 90.00

Refinement procedure

Resolution	40.000 - 2.000
R-factor	0.204
Rwork	0.204
R-free	0.25900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	THE STARTING STRUCTURAL MODEL WAS DETERMINED FROM A CRYSTAL GROWN WITH THE RECOMBINANT SINGLY-IODINATED PROTEIN ISOFORM HPLC-6. THIS MODEL IS NOT YET AVAILABLE.
RMSD bond length	0.014
RMSD bond angle	27.200 *
Data reduction software	R-AXIS
Data scaling software	R-AXIS
Phasing software	X-PLOR (3.843)
Refinement software	X-PLOR (3.843)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000
High resolution limit [Å]	2.000
Rmerge	0.066 *
Number of reflections	3107
Completeness [%]	82.4	54.9 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8		pH 8.0

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	8 (mg/ml)
2	1	reservoir	Tris-HCl	68 (mM)
3	1	reservoir	ammonium sulfate	2.72 (M)