1OPF

THE STRUCTURE OF OMPF PORIN IN A TETRAGONAL CRYSTAL FORM

Experimental procedure

Spacegroup name	P 42
Unit cell lengths	154.900, 154.900, 172.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 3.200
R-factor	0.241
Rwork	0.241
RMSD bond length	0.010
RMSD bond angle	1.581
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	3.200 *
Rmerge	0.125 *
Total number of observations	133465 *
Number of reflections	56516
Completeness [%]	87.0
Redundancy	2.4 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *		21-23 *	Garavito, R.M., (1986) Methods Enzymol., 125, 309. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	6-9 (mg/ml)
10	1	reservoir		0.01 (%)
2	1	drop	PEG2000	7.5 (%(w/v))
3	1	drop	sodium phosphate	20-50 (mM)
4	1	drop	octylglucoside	0.8 (%(w/v))
5	1	drop	detergent	0.08 (%(w/v))
6	1	drop		0.01 (%(w/v))
7	1	reservoir	PEG2000	15 (%)
8	1	reservoir		40-500 (mM)
9	1	reservoir	sodium phosphate	40-100 (mM)