1OPE
Deletion mutant of SUCCINYL-COA:3-KETOACID COA TRANSFERASE FROM PIG HEART
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-09-18 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.00 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 147.640, 68.700, 103.500 |
| Unit cell angles | 90.00, 99.58, 90.00 |
Refinement procedure
| Resolution | 38.130 - 2.500 |
| R-factor | 0.18 |
| Rwork | 0.180 |
| R-free | 0.23500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ooy |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.000 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.040 | 0.086 |
| Number of reflections | 30903 | |
| <I/σ(I)> | 18 | 11.1 |
| Completeness [%] | 85.5 | 86.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | PEG 2000, sodium/potassium phosphate, sodium azide , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
