1OP9

Complex of human lysozyme with camelid VHH HL6 antibody fragment

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2001-03-01
Detector	MARRESEARCH
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	39.611, 48.226, 66.605
Unit cell angles	90.00, 98.98, 90.00

Refinement procedure

Resolution	31.000 - 1.860
R-factor	0.197
Rwork	0.196
R-free	0.23800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	antibody: 1jto lysozyme: 133l
RMSD bond length	0.008
RMSD bond angle	0.023
Data reduction software	DENZO
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.930
High resolution limit [Å]	1.860	1.860
Rmerge	0.066	0.229
Number of reflections	20015
<I/σ(I)>	13.8	5.7
Completeness [%]	95.3	89
Redundancy	4.4	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	20 *	20 % W/V PEG4000, 0.2M imidazole malate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	0.2 (mM)
2	1	reservoir	PEG4000	20 (%(w/v))
3	1	reservoir	imidazole-malate	0.2 (M)	pH6.0