1OOY
SUCCINYL-COA:3-KETOACID COA TRANSFERASE FROM PIG HEART
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1998-07-10 |
| Detector | BRANDEIS |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 99.449, 140.325, 68.095 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.810 - 1.700 |
| R-factor | 0.156 |
| Rwork | 0.156 |
| R-free | 0.19400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1m3e |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.000 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.059 | 0.216 |
| Number of reflections | 100807 | |
| <I/σ(I)> | 20.6 | 2.5 |
| Completeness [%] | 90.7 | 73.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | PEG 2000, sodium/potassium phosphate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
