1OOT
Crystal structure of the SH3 domain from a S. cerevisiae hypothetical 40.4 kDa protein at 1.39 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X13 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-03-01 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.8028 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 39.930, 39.930, 69.490 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 1.390 |
| R-factor | 0.13397 |
| Rwork | 0.132 |
| R-free | 0.17046 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1sem |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.842 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.27) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.440 |
| High resolution limit [Å] | 1.380 | 1.380 |
| Number of reflections | 13185 | |
| <I/σ(I)> | 15 | 2.7 |
| Completeness [%] | 97.0 | 88.1 |
| Redundancy | 5.5 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 295 | 70% MPD, pH 7.5, VAPOR DIFFUSION, temperature 295K |
