1OOP

The Crystal Structure of Swine Vesicular Disease Virus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX14.2
Synchrotron site	SRS
Beamline	PX14.2
Temperature [K]	298
Detector technology	CCD
Collection date	2000-02-29
Detector	ADSC QUANTUM 4
Wavelength(s)	0.979
Spacegroup name	P 2 21 21
Unit cell lengths	354.100, 371.700, 318.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 3.000
R-factor	0.245
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Coxsackievirus A9
RMSD bond length	0.018
RMSD bond angle	2.200
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	3.110
High resolution limit [Å]	3.000	3.000
Rmerge	0.207	0.482
Number of reflections	406689
<I/σ(I)>	5.1
Completeness [%]	49.2	46 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.6	298	15-25% saturated ammonium sulfate, 100mM phosphate buffer, pH 7.6, VAPOR DIFFUSION, SITTING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	ammonium sulfate	15-25 (%sat)
3	1	reservoir	phosphate buffer	100 (mM)	pH7.6