1ONX
Crystal structure of isoaspartyl dipeptidase from escherichia coli complexed with aspartate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 277 |
Detector technology | AREA DETECTOR |
Collection date | 2003-01-20 |
Detector | SIEMENS HI-STAR |
Wavelength(s) | 1.5418 |
Spacegroup name | P 4 21 2 |
Unit cell lengths | 119.100, 119.100, 138.100 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.100 |
R-factor | 0.182 |
Rwork | 0.177 |
R-free | 0.24600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1onw |
RMSD bond length | 0.013 |
RMSD bond angle | 17.100 * |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | AMoRE |
Refinement software | TNT (5E) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.200 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.082 * | 0.263 * |
Number of reflections | 55201 | 5640 * |
<I/σ(I)> | 6.7 | 1.6 |
Completeness [%] | 94.5 | 75.4 |
Redundancy | 4.2 | 1.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 4 * | PEG8000, homopipes, magnesium chloride, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | PEG8000 | 6-8 (%) | |
2 | 1 | reservoir | homopipes | 100 (mM) | pH5.0 |
3 | 1 | reservoir | 50-100 (mM) | ||
4 | 1 | drop | protein | 11.0 (mg/ml) | |
5 | 1 | drop | Tris | 50 (mM) | pH8.1 |