1ONS
Crystal structure of Escherichia coli heat shock protein YedU
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CAMD BEAMLINE GCPCC |
| Synchrotron site | CAMD |
| Beamline | GCPCC |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-11-12 |
| Detector | MACSCIENCE |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 52.980, 52.980, 347.400 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.200 |
| R-factor | 0.22 |
| Rwork | 0.204 |
| R-free | 0.24300 * |
| Structure solution method | MIR |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 90.000 | 2.250 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.075 | 0.319 * |
| Total number of observations | 290299 * | |
| Number of reflections | 15151 | 779 * |
| <I/σ(I)> | 14.7 | 3.9 |
| Completeness [%] | 94.5 | 77.5 |
| Redundancy | 5 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.2 * | 298 | PEG 600 and ammonium sulfate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15.0 (mg/ml) | |
| 2 | 1 | drop | dithiothreitol | 5 (mM) | |
| 3 | 1 | drop | 10 (mM) | ||
| 4 | 1 | drop | Tris | 20 (mM) | pH8.2 |
| 5 | 1 | reservoir | PEG600 | 2 (%) | |
| 6 | 1 | reservoir | dithiothreitol | 5 (mM) | |
| 7 | 1 | reservoir | ammonium sulfate | 1.8 (M) | |
| 8 | 1 | reservoir | HEPES | 0.1 (M) | pH7.5 |
