1ONL

Crystal structure of Thermus thermophilus HB8 H-protein of the glycine cleavage system

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44B2
Synchrotron site	SPring-8
Beamline	BL44B2
Temperature [K]	100
Detector technology	CCD
Collection date	2001-06-22
Detector	MARRESEARCH
Wavelength(s)	1.00
Spacegroup name	P 65
Unit cell lengths	55.775, 55.775, 191.190
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 * - 2.500
R-factor	0.186
Rwork	0.186
R-free	0.25600 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1hpc
RMSD bond length	0.006
RMSD bond angle	24.400 *
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	EPMR
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000 *	2.580
High resolution limit [Å]	2.500	2.500
Rmerge	0.109	0.355
Total number of observations	135290 *
Number of reflections	11730
<I/σ(I)>	13.7	2.5
Completeness [%]	99.6 *	98.5
Redundancy	11.5	5.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8 *	291	ammonium phosphate, glycerol, pH 4.3, VAPOR DIFFUSION, HANGING DROP, temperature 291K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	Tris-HCl	20 (mM)	pH8.0
3	1	drop		150 (mM)
4	1	drop	dithiothreitol	1 (mM)
5	1	reservoir	ammonium phosphate	0.4 (M)
6	1	reservoir	glycerol	24 (%(v/v))	pH4.3