1ONA
CO-CRYSTALS OF CONCANAVALIN A WITH METHYL-3,6-DI-O-(ALPHA-D-MANNOPYRANOSYL)-ALPHA-D-MANNOPYRANOSIDE
Experimental procedure
| Source type | ROTATING ANODE |
| Temperature [K] | 100 |
| Detector technology | DIFFRACTOMETER |
| Collection date | 1996-03-13 |
| Detector | ENRAF-NONIUS FAST |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 59.830, 64.840, 125.920 |
| Unit cell angles | 90.00, 93.87, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.350 |
| R-factor | 0.221 |
| Rwork | 0.221 |
| R-free | 0.28200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5cna |
| RMSD bond length | 0.012 |
| RMSD bond angle | 26.520 * |
| Data reduction software | MADNES |
| Data scaling software | CCP4 |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 8.000 * | 2.420 |
| High resolution limit [Å] | 2.350 * | 2.300 |
| Rmerge | 0.065 * | 0.197 * |
| Total number of observations | 44422 * | |
| Number of reflections | 28287 * | |
| <I/σ(I)> | 8.3 | 3.8 |
| Completeness [%] | 73.0 * | 22.6 |
| Redundancy | 1.5 | 1.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.5 | 13% POLYETHYLENEGLYCOLMETHYLETHER, 40 MM HEPES PH 6.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 8 (mg/ml) | |
| 2 | 1 | drop | 250 (mM) | ||
| 3 | 1 | drop | 1 (mM) | ||
| 4 | 1 | drop | 1 (mM) | ||
| 5 | 1 | drop | 0.02 (%) | ||
| 6 | 1 | drop | trisaccharide | 12.5 (mM) | |
| 7 | 1 | reservoir | mPEG5000 | 13 (%) | |
| 8 | 1 | reservoir | HEPES | 40 (mM) |
