1ON7
Unmethylated form of C-phycocyanin from Themosynechococcus vulcanus at 2.7A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-12-15 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.8126 |
| Spacegroup name | P 63 |
| Unit cell lengths | 153.290, 153.290, 39.048 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.700 |
| Rwork | 0.209 |
| R-free | 0.27800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ktp |
| RMSD bond length | 0.009 * |
| RMSD bond angle | 1.260 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.065 | 0.550 |
| Number of reflections | 12540 | |
| <I/σ(I)> | 7.4 | 1.2 |
| Completeness [%] | 84.7 | 70.2 |
| Redundancy | 2.4 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 293 | PEG4000, Bis-Tris, 6.3mg/ml protein, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | HEPES | 20 (mM) | pH8.0 |
| 2 | 1 | drop | protein | 20 (mg/ml) | |
| 3 | 1 | drop | PEG4000 | 6.7 (%) | |
| 4 | 1 | drop | PC612 | 6.3 (mg/ml) | |
| 5 | 1 | drop | Bis-Tris | 70 (mM) | pH7.0 |
| 6 | 1 | reservoir | PEG4000 | 10 (%) | |
| 7 | 1 | reservoir | bis-Tris | 100 (mM) | pH7.0 |
