1ON1
Bacillus Subtilis Manganese Transport Regulator (Mntr) Bound To Manganese, AB Conformation.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL1-5 |
| Synchrotron site | SSRL |
| Beamline | BL1-5 |
| Temperature [K] | 100 |
| Wavelength(s) | 0.98397 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.120, 46.020, 73.560 |
| Unit cell angles | 90.00, 92.72, 90.00 |
Refinement procedure
| Resolution | 40.500 - 1.750 |
| Rwork | 0.216 |
| R-free | 0.24600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bi2 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.980 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.500 | 1.800 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.048 | 0.193 |
| Total number of observations | 107792 * | |
| Number of reflections | 32650 | |
| <I/σ(I)> | 8.5 | 2.1 |
| Completeness [%] | 96.3 | 90.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 30% PEG 400, 0.15 M Lithium sulfate, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | reservoir | Tris-HCl | 0.1 (M) | pH8.5 |
| 3 | 1 | reservoir | PEG400 | 20-30 (%(v/v)) | |
| 4 | 1 | reservoir | 0.15 (M) | ||
| 5 | 1 | reservoir | 2 (mM) |
