1OMV
non-myristoylated bovine recoverin (E85Q mutant) with calcium bound to EF-hand 3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-04-21 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.934 |
| Spacegroup name | I 4 |
| Unit cell lengths | 84.333, 84.333, 58.809 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 59.630 - 1.900 |
| R-factor | 0.24 |
| Rwork | 0.240 |
| R-free | 0.24900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1omr |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.383 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.760 | 1.840 |
| High resolution limit [Å] | 1.900 * | 1.800 |
| Rmerge | 0.087 * | 0.208 * |
| Number of reflections | 16253 * | |
| <I/σ(I)> | 4.7 | 3.1 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 290 | ammonium sulfate, TRIS, calcium chloride, magnesium chloride, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Tris-HCl | 100 (mM) | pH8.0 |
| 2 | 1 | drop | 1 (mM) | ||
| 3 | 1 | drop | 1 (mM) | ||
| 4 | 1 | reservoir | ammonium sulfate | 70-80 (%sat) |
