1OMR

non-myristoylated wild-type bovine recoverin with calcium bound to EF-hand 3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2002-04-21
Detector	ADSC QUANTUM 4
Wavelength(s)	0.934
Spacegroup name	I 4
Unit cell lengths	84.051, 84.051, 59.179
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.390 - 1.500
R-factor	0.249
Rwork	0.249
R-free	0.25000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1rec
RMSD bond length	0.007
RMSD bond angle	1.329 *
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.390	1.540
High resolution limit [Å]	1.500	1.500
Rmerge	0.068 *	0.422 *
Number of reflections	33042
<I/σ(I)>	5.2	2
Completeness [%]	99.9	100
Redundancy	3.8	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	290	ammonium sulfate, TRIS, calcium chloride, magnesium chloride, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 290K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	Tris-HCl	100 (mM)	pH8.0
2	1	drop		1 (mM)
3	1	drop		1 (mM)
4	1	reservoir	ammonium sulfate	70-80 (%sat)