1OMP

CRYSTALLOGRAPHIC EVIDENCE OF A LARGE LIGAND-INDUCED HINGE-TWIST MOTION BETWEEN THE TWO DOMAINS OF THE MALTODEXTRIN-BINDING PROTEIN INVOLVED IN ACTIVE TRANSPORT AND CHEMOTAXIS

Experimental procedure

Spacegroup name	P 1
Unit cell lengths	38.350, 44.440, 58.250
Unit cell angles	101.00, 100.40, 104.20

Refinement procedure

Resolution	10.000 - 1.800
R-factor	0.21
Rwork	0.210
RMSD bond length	0.015
RMSD bond angle	0.031 *
Phasing software	X-PLOR
Refinement software	PROLSQ

Data quality characteristics

	Overall
High resolution limit [Å]	1.730 *
Rmerge	0.057 *
Total number of observations	107789 *
Number of reflections	33722 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.2 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	PEG2000	13 (%)
2	1	drop	protein	6 (mg/ml)
3	1	drop	MES	20 (mM)
4	1	reservoir	PEG2000	30 (%)