1OMO
alanine dehydrogenase dimer w/bound NAD (archaeal)
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X26C |
| Synchrotron site | NSLS |
| Beamline | X26C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-04-11 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.088,1.1048,1.1051 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 108.700, 55.500, 131.300 |
| Unit cell angles | 90.00, 111.10, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.320 |
| R-factor | 0.213 |
| Rwork | 0.203 |
| R-free | 0.26200 |
| Structure solution method | MAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.890 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.400 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.040 | |
| Number of reflections | 32447 | |
| <I/σ(I)> | 17.6 | |
| Completeness [%] | 98.0 | 98 |
| Redundancy | 3.8 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.9 | 295 | Protein 15 mg/mL, MPD (~20%), 100 mM NaCl, 10 mM Tris, pH 4.9, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
