1OMK
The Crystal Structure of d(CACG(5IU)G)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-05-05 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 18.155, 30.034, 41.988 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | NDB ENTRY ZDFB51 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | SHELXL |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.350 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.062 | 0.165 |
| Total number of observations | 137352 * | |
| Number of reflections | 5988 | |
| Completeness [%] | 98.2 | 98.8 |
| Redundancy | 22.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.9 * | 290 | potassium cacodylate, MPD, magnesium chloride, potassium chloride, cobalt hexamine , pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 290.0K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | potassium cacodylate | ||
| 2 | 1 | 1 | MPD | ||
| 3 | 1 | 1 | magnesium chloride | ||
| 4 | 1 | 1 | potassium chloride | ||
| 5 | 1 | 1 | cobalt hexamine | ||
| 6 | 1 | 2 | MPD | ||
| 7 | 1 | 2 | potassium cacodylate | ||
| 8 | 1 | 2 | magnesium chloride | ||
| 9 | 1 | 2 | potassium chloride |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 7.66 (mg/ml) | |
| 2 | 1 | drop | potassium cacodylate | 28.7 (mM) | pH6.9 |
| 3 | 1 | drop | MPD | 24.5 (%(v/v)) | |
| 4 | 1 | reservoir | potassium cacodylate | 40 (mM) | pH5.5 |
| 5 | 1 | reservoir | cobalt hexammine | 6 (mM) | |
| 6 | 1 | reservoir | 80 (mM) | ||
| 7 | 1 | reservoir | 20 (mM) | ||
| 8 | 1 | reservoir | MPD | 10 (%(v/v)) |
