1OMI
Crystal structure of PrfA,the transcriptional regulator in Listeria monocytogenes
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X9A |
| Synchrotron site | NSLS |
| Beamline | X9A |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2001-06-10 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 69.430, 72.091, 114.335 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.800 |
| R-factor | 0.248 |
| Rwork | 0.244 |
| R-free | 0.27800 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.072 | 0.299 |
| Number of reflections | 16444 | |
| <I/σ(I)> | 28.6 | 7.2 |
| Completeness [%] | 98.0 | 95.2 |
| Redundancy | 9.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.5 | 319 | 20% ethanol, 0.1M tris HCl, pH 8.5, VAPOR DIFFUSION, temperature 319K |
