1OME
CRYSTAL STRUCTURE OF THE OMEGA LOOP DELETION MUTANT (RESIDUES 163-178 DELETED) OF BETA-LACTAMASE FROM STAPHYLOCOCCUS AUREUS PC1
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | SIEMENS |
| Temperature [K] | 298 |
| Detector technology | AREA DETECTOR |
| Collection date | 1996-05 |
| Detector | SIEMENS |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 76.000, 55.000, 79.200 |
| Unit cell angles | 90.00, 90.25, 90.00 |
Refinement procedure
| Resolution | 7.000 - 2.300 |
| R-factor | 0.19 |
| Rwork | 0.190 |
| R-free | 0.29800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3blm |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.400 * |
| Data reduction software | XENGEN |
| Data scaling software | XENGEN |
| Phasing software | X-PLOR (3.8) |
| Refinement software | X-PLOR (3.8) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 2.440 | |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.112 | 0.320 |
| Number of reflections | 25484 | |
| <I/σ(I)> | 10 | 2 |
| Completeness [%] | 86.7 | 70 |
| Redundancy | 2 | 1.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8 | 68% SATURATED AMMONIUM SULFATE, 0.5% PEG 1000, 0.3M KCL, 0.1M SODIUM BICARBONATE PH 8.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | enzyme | 4.0 (mg/ml) | |
| 2 | 1 | drop | ammonium sulfate | 34 (%sat) | |
| 3 | 1 | drop | PEG1000 | 0.25 (%) | |
| 4 | 1 | drop | potassium chloride | 0.15 (M) | |
| 5 | 1 | drop | sodium bicarbonate | 0.05 (M) | |
| 6 | 1 | reservoir | ammonium sulfate | 68 (%sat) | |
| 7 | 1 | reservoir | PEG1000 | 0.5 (%) | |
| 8 | 1 | reservoir | potassium chloride | 0.3 (M) | |
| 9 | 1 | reservoir | sodium bicarbonate | 0.1 (M) |
