1OM6

CRYSTAL STRUCTURE OF A COLD ADAPTED ALKALINE PROTEASE FROM PSEUDOMONAS TAC II 18, CO-CRYSTALLIZED WITH 5mM EDTA (2 MONTHS)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM30A
Synchrotron site	ESRF
Beamline	BM30A
Temperature [K]	100
Detector technology	CCD
Collection date	2002-04-04
Detector	MARRESEARCH
Wavelength(s)	0.98
Spacegroup name	H 3
Unit cell lengths	180.500, 180.500, 37.350
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 2.000
R-factor	0.188
Rwork	0.185
R-free	0.22900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1g9k
RMSD bond length	0.011
RMSD bond angle	1.620
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.100	2.110
High resolution limit [Å]	2.000	2.000
Rmerge	0.081 *	0.167 *
Total number of observations	123884 *
Number of reflections	30649
Completeness [%]	100.0	100
Redundancy	4	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	4 *	Villeret, V., (1997) Protein Sci., 6, 2462. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium sulfate	1.6 (M)
2	1	reservoir	HEPES	0.1 (M)	pH7.0
3	1	drop	protein	15 (mg/ml)