1OLL

Extracellular region of the human receptor NKp46

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE BW7B
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	BW7B
Temperature [K]	100
Detector technology	CCD
Collection date	2002-06-15
Detector	MARRESEARCH
Spacegroup name	P 61
Unit cell lengths	85.477, 85.477, 59.909
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.000 - 1.930
R-factor	0.198
Rwork	0.195
R-free	0.25100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1g0x
RMSD bond length	0.011 *
RMSD bond angle	0.952 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	1.960
High resolution limit [Å]	1.930	1.930
Rmerge	0.075	0.765
Number of reflections	18944
<I/σ(I)>	22.9	2.4
Completeness [%]	100.0	100
Redundancy	5	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7.8	277	15% PEG 8000, 100 MM HEPES PH 7.8, 20% ETHANEDIOL, AT 277 K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	HEPES	100 (mM)	pH7.8
2	1	reservoir	PEG8000	15 (%)
3	1	reservoir	ethanediol	20 (%(v/v))