1OKI

Crystal structure of truncated human beta-B1-crystallin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-3
Synchrotron site	ESRF
Beamline	ID14-3
Temperature [K]	100
Detector technology	CCD
Detector	MARRESEARCH
Spacegroup name	P 43
Unit cell lengths	45.040, 45.040, 171.111
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.170 - 1.400
R-factor	0.172
Rwork	0.171
R-free	0.21100 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2bb2
RMSD bond length	0.023 *
RMSD bond angle	1.870 *
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	REFMAC (5.1.29)

Data quality characteristics

	Overall
Low resolution limit [Å]	35.000
High resolution limit [Å]	1.400
Rmerge	0.043
Total number of observations	315295 *
Number of reflections	60531
<I/σ(I)>	8.9
Completeness [%]	85.6
Redundancy	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Batch method *	8 *		Bateman, O.A., (2001) Exp. Eye Res., 73, 321. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	Tris-HCl	25 (mM)	pH8.0
2	1	1	dithiothreitol	1 (mM)
3	1	1	protein	10 (mg/ml)