1OKH
Viscotoxin A3 from Viscum album L.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X13 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-04-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 47.995, 68.593, 25.264 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.750 |
| R-factor | 0.1887 |
| R-free | 0.23900 * |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.021 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXD |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 68.590 | 1.850 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.089 | 0.387 |
| Total number of observations | 50787 * | |
| Number of reflections | 8938 | |
| <I/σ(I)> | 14.31 | 3.89 |
| Completeness [%] | 100.0 | 99.7 |
| Redundancy | 5.3 * | 4.4 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.5 | 0.15M AM2SO4, 0.05M CACOD. PH=6.5, 30% PEG8000, 15MM HGCL2, pH 6.50 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15 (mg/ml) | |
| 2 | 1 | reservoir | ammonium sulfate | 0.15 (M) | |
| 3 | 1 | reservoir | cacodylate | 0.05 (M) | pH6.5 |
| 4 | 1 | reservoir | PEG8000 | 30 (%) | |
| 5 | 1 | reservoir | 15 (mM) |
