1OKB
crystal structure of Uracil-DNA glycosylase from Atlantic cod (Gadus morhua)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM1A |
| Synchrotron site | ESRF |
| Beamline | BM1A |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-02-15 |
| Detector | MAR scanner 345 mm plate |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 68.580, 67.189, 68.644 |
| Unit cell angles | 90.00, 119.86, 90.00 |
Refinement procedure
| Resolution | 12.000 - 1.900 |
| R-factor | 0.1861 |
| Rwork | 0.186 |
| R-free | 0.20570 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1akz |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.189 |
| Data reduction software | DENZO |
| Data scaling software | SCALA |
| Phasing software | CNS |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 12.000 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.096 | 0.522 |
| Number of reflections | 42162 | |
| <I/σ(I)> | 10.2 | 2 |
| Completeness [%] | 98.7 | 97.5 |
| Redundancy | 3.3 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.1M HEPES PH 7.5, 1.4M SODIUM CITRATE |
