1OK4
Archaeal fructose 1,6-bisphosphate aldolase covalently bound to the substrate dihydroxyacetone phosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 100 |
| Collection date | 2002-02-15 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 82.400, 157.300, 101.500 |
| Unit cell angles | 90.00, 108.20, 90.00 |
Refinement procedure
| Resolution | 40.000 * - 2.100 |
| R-factor | 0.164 |
| Rwork | 0.163 |
| R-free | 0.18700 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ojx |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.300 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.1.19) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.190 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.071 | 0.272 |
| Number of reflections | 125363 | |
| <I/σ(I)> | 13.6 | 2.3 |
| Completeness [%] | 90.2 | 59.3 |
| Redundancy | 3.6 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 * | 20 * | 9% (W/V) PEG 4000, 0.1 M NA ACETATE PH 5.0, 1-8% GLYCEROL, 100 MM DHAP |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 3 (mg/ml) | |
| 2 | 1 | drop | HEPES-NaOH | 10 (mM) | pH7.5 |
| 3 | 1 | drop | 100 (mM) | ||
| 4 | 1 | drop | dithiothreitol | 1 (mM) | |
| 5 | 1 | reservoir | PEG4000 | 9 (%(w/v)) | |
| 6 | 1 | reservoir | sodium acetate | 0.1 (M) | pH5.0 |
