1OJY
Decay accelerating factor (cd55): the structure of an intact human complement regulator.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-07-15 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 |
| Unit cell lengths | 51.829, 78.694, 80.903 |
| Unit cell angles | 72.70, 79.38, 81.70 |
Refinement procedure
| Resolution | 25.000 * - 2.600 |
| R-free | 0.29700 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ojv |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.700 * |
| Data reduction software | DENZO (V. 1.96)) |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | TNT (5F) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.600 * | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.089 | 0.202 |
| Number of reflections | 34638 | |
| <I/σ(I)> | 8.9 | 1.9 |
| Completeness [%] | 94.4 | 94.2 |
| Redundancy | 2.4 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 4.6 | 4 * | 0.2 M AMMONIUM SULPHATE 20% M-PEG 5K, 0.1M SODIUM ACETATE PH 4.6, 10% GLYCEROL |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | ammonium sulfate | 0.2 (M) | |
| 2 | 1 | reservoir | MPEG5000 | 20 (%(w/v)) | |
| 3 | 1 | reservoir | sodium acetate | 0.1 (M) | pH4.6 |
| 4 | 1 | reservoir | glycerol | 10 (%(w/v)) |
