1OJY

Decay accelerating factor (cd55): the structure of an intact human complement regulator.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	100
Detector technology	CCD
Collection date	2001-07-15
Detector	ADSC CCD
Spacegroup name	P 1
Unit cell lengths	51.829, 78.694, 80.903
Unit cell angles	72.70, 79.38, 81.70

Refinement procedure

Resolution	25.000 * - 2.600
R-free	0.29700 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ojv
RMSD bond length	0.005
RMSD bond angle	0.700 *
Data reduction software	DENZO (V. 1.96))
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	TNT (5F)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.600 *	2.690
High resolution limit [Å]	2.600	2.600
Rmerge	0.089	0.202
Number of reflections	34638
<I/σ(I)>	8.9	1.9
Completeness [%]	94.4	94.2
Redundancy	2.4 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	4.6	4 *	0.2 M AMMONIUM SULPHATE 20% M-PEG 5K, 0.1M SODIUM ACETATE PH 4.6, 10% GLYCEROL

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium sulfate	0.2 (M)
2	1	reservoir	MPEG5000	20 (%(w/v))
3	1	reservoir	sodium acetate	0.1 (M)	pH4.6
4	1	reservoir	glycerol	10 (%(w/v))