1OJT
STRUCTURE OF DIHYDROLIPOAMIDE DEHYDROGENASE
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | LURE |
| Synchrotron site | LURE |
| Temperature [K] | 277 |
| Detector technology | IMAGE PLATE |
| Detector | MAR scanner 300 mm plate |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 138.050, 138.050, 79.770 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 5.000 * - 2.750 |
| R-factor | 0.173 |
| R-free | 0.23200 |
| Structure solution method | MOLECULAR REPLACEMENT AND MIR |
| Starting model (for MR) | DLDH OF PSEUDOMONAS PUTIDA PDB ENTRY 1LVL. |
| RMSD bond length | 0.020 |
| RMSD bond angle | 0.050 |
| Data reduction software | MOSFLM ((+ CCP4)) |
| Data scaling software | CCP4 ((SCALEIT)) |
| Phasing software | SHARP |
| Refinement software | PROLSQ |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.900 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.066 | 0.190 |
| Number of reflections | 11724 | |
| <I/σ(I)> | 18 | 4 |
| Completeness [%] | 94.0 | 70 |
| Redundancy | 7 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 * | Li De La Sierra, I., (1994) J.Mol.Biol., 235, 1154. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15-20 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 0.02 (M) | |
| 3 | 1 | drop | EDTA | 0.006 (M) | |
| 4 | 1 | drop | 0.15 (M) | ||
| 5 | 1 | reservoir | potassium phosphate | 0.1 (M) | |
| 6 | 1 | reservoir | ammonium sulfate | 2 (M) |
