1OIW
X-ray structure of the small G protein Rab11a in complex with GTPgammaS
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-2 |
Synchrotron site | ESRF |
Beamline | ID14-2 |
Temperature [K] | 100 |
Collection date | 2002-05-17 |
Spacegroup name | I 4 2 2 |
Unit cell lengths | 73.726, 73.726, 125.152 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.300 * |
R-factor | 0.265 |
Rwork | 0.265 |
R-free | 0.23300 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1oiv |
RMSD bond length | 0.016 |
RMSD bond angle | 24.000 * |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.096 | 0.288 |
Total number of observations | 207365 * | |
Number of reflections | 13984 * | |
<I/σ(I)> | 22.16 | 9.35 |
Completeness [%] | 96.5 | 81.6 |
Redundancy | 14.9 | 14 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 6.5 | 20 * | 1.4 M NACL, 0.15 M NAH2PO4, 0.15 M KH2PO4,0.1 M NAMES PH6.5, pH 6.50 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | drop | DTPgammaS | 5 (mM) | |
3 | 1 | reservoir | 1.1-1.5 (M) | ||
4 | 1 | reservoir | 150-200 (mM) | ||
5 | 1 | reservoir | 150-200 (mM) | ||
6 | 1 | reservoir | sodium-MES | 100 (%(w/v)) | pH6.5 |