1OIW

X-ray structure of the small G protein Rab11a in complex with GTPgammaS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	100
Collection date	2002-05-17
Spacegroup name	I 4 2 2
Unit cell lengths	73.726, 73.726, 125.152
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.300 *
R-factor	0.265
Rwork	0.265
R-free	0.23300 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1oiv
RMSD bond length	0.016
RMSD bond angle	24.000 *
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.970
High resolution limit [Å]	1.900	1.900
Rmerge	0.096	0.288
Total number of observations	207365 *
Number of reflections	13984 *
<I/σ(I)>	22.16	9.35
Completeness [%]	96.5	81.6
Redundancy	14.9	14

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	6.5	20 *	1.4 M NACL, 0.15 M NAH2PO4, 0.15 M KH2PO4,0.1 M NAMES PH6.5, pH 6.50

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	DTPgammaS	5 (mM)
3	1	reservoir		1.1-1.5 (M)
4	1	reservoir		150-200 (mM)
5	1	reservoir		150-200 (mM)
6	1	reservoir	sodium-MES	100 (%(w/v))	pH6.5