1OI7
The Crystal Structure of Succinyl-CoA synthetase alpha subunit from Thermus Thermophilus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-05-15 |
| Detector | RIGAKU RAXIS V |
| Spacegroup name | P 31 |
| Unit cell lengths | 61.540, 61.540, 60.877 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 1.230 |
| R-factor | 0.178 |
| Rwork | 0.178 |
| R-free | 0.19600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1scu |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.270 |
| High resolution limit [Å] | 1.230 | 1.230 |
| Rmerge | 0.023 | 0.190 |
| Number of reflections | 74888 | |
| <I/σ(I)> | 34.9 | 10.3 |
| Completeness [%] | 99.7 | 99.5 |
| Redundancy | 4.1 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.6 | PROTEIN WAS CRYSTALLIZED FROM 22.5% PEG 4000, 100 MM CHES,, pH 8.60 |
