1OI3
X-ray structure of the dihydroxyacetone kinase from Escherichia coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Temperature [K] | 100 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 96.465, 97.364, 86.213 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.730 - 2.000 |
| R-factor | 0.189 |
| Rwork | 0.189 |
| R-free | 0.23900 |
| Structure solution method | OTHER |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.780 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC (5.1.19) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.040 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.046 | 0.100 |
| Number of reflections | 51496 | |
| <I/σ(I)> | 23.7 | |
| Completeness [%] | 93.1 | 60.6 |
| Redundancy | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5 | pH 5.00 |
