1OHT
Peptidoglycan recognition protein LB
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 110 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 40.561, 40.561, 337.997 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.000 |
| R-factor | 0.216 |
| Rwork | 0.216 |
| R-free | 0.24300 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.711 |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | |
| High resolution limit [Å] | 2.000 | |
| Rmerge | 0.071 * | 0.201 * |
| Number of reflections | 12443 | |
| Completeness [%] | 91.7 | 88.2 * |
| Redundancy | 44 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6 | 14 * | pH 6.00 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | potassium sodium tartrate | 1.0 (M) | |
| 2 | 1 | reservoir | 2-morpholinoethane sulfonic acid | 0.1 (M) | pH6.0 |
