1OHA
Acetylglutamate kinase from Escherichia coli complexed with MgADP and N-acetyl-L-glutamate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-05-10 |
| Detector | MARRESEARCH |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 60.126, 71.792, 107.175 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.430 - 1.900 |
| R-factor | 0.2 |
| Rwork | 0.200 |
| R-free | 0.22950 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gs5 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.500 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.430 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.068 | 0.469 |
| Total number of observations | 154986 * | |
| Number of reflections | 18636 | |
| <I/σ(I)> | 7.3 | 1.3 |
| Completeness [%] | 99.9 | 99.6 |
| Redundancy | 8.3 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 4.6 | 27-29% POLYETHYLENE GLYCOL MONOMETHYL ETHER 2K, SODIUM ACETATE 0.1M PH 4.6 AMMONIUM SULFATE 0.15-0.3 M |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG2000 | 27-29 (%(w/v)) | |
| 2 | 1 | reservoir | sodium acetate | 0.1 (M) | pH4.6 |
| 3 | 1 | reservoir | ammonium citrate | 0.25-0.4 (M) | |
| 4 | 1 | drop | protein | 10 (mg/ml) |
