1OH8
THE CRYSTAL STRUCTURE OF E. COLI MUTS BINDING TO DNA WITH AN UNPAIRED THYMIDINE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-12-15 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 89.381, 91.719, 259.694 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.900 |
| R-factor | 0.221 |
| Rwork | 0.217 |
| R-free | 0.29200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1e3m |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.261 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 * | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.124 | 0.725 |
| Total number of observations | 332212 * | |
| Number of reflections | 48882 * | |
| <I/σ(I)> | 9.54 | 2.2 |
| Completeness [%] | 98.3 | 92.4 |
| Redundancy | 6.7 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 * | 12-14 % PEG 6000, 150-300 MM NACL 25 MM HEPES PH 7-8, 10 MM MGCL2, 100-150 MICROM ADP |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG6000 | 11-14 (%) | |
| 2 | 1 | reservoir | 350-750 (mM) | ||
| 3 | 1 | reservoir | 10 (mM) | ||
| 4 | 1 | reservoir | HEPES | 25 (mM) | pH7.5 |
| 5 | 1 | drop | protein | 14 (mg/ml) |
