1OGQ

The crystal structure of PGIP (polygalacturonase inhibiting protein), a leucine rich repeat protein involved in plant defense

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ELETTRA BEAMLINE 5.2R
Synchrotron site	ELETTRA
Beamline	5.2R
Temperature [K]	100
Detector technology	CCD
Collection date	2001-05-15
Detector	MARRESEARCH
Spacegroup name	P 21 21 2
Unit cell lengths	134.840, 65.450, 34.640
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.000 - 1.700
Rwork	0.190
R-free	0.24400 *
Structure solution method	SIRAS
RMSD bond length	0.015 *
RMSD bond angle	2.600 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000
High resolution limit [Å]	1.700	1.700
Rmerge	0.064	0.450
Total number of observations	330460 *
Number of reflections	34715
<I/σ(I)>	11.4
Completeness [%]	94.1	65.5
Redundancy	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	4.6	4 *	pH 4.60

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG4000	20-30 (%(w/v))
2	1	reservoir	ammonium acetate	0.18-0.2 (M)
3	1	reservoir	sodium acetate	0.1 (M)	pH4.6
4	1	drop	protein	4.0 (mg/ml)