1OGG
chitinase b from serratia marcescens mutant d142n in complex with inhibitor allosamidin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Collection date | 2002-04-26 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 55.866, 104.744, 186.728 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.760 - 1.970 |
| R-factor | 0.198 |
| Rwork | 0.198 |
| R-free | 0.23800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1e6r |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.500 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.040 |
| High resolution limit [Å] | 1.970 | 1.970 |
| Rmerge | 0.043 | 0.330 |
| Number of reflections | 19590 | |
| <I/σ(I)> | 21.7 | 3 |
| Completeness [%] | 95.3 | 87.9 |
| Redundancy | 3.2 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 15 % GLYCEROL 100 MM HEPES, PH 7, 1.3 M AMMONIUM SULPHATE |
