1OGA

A structural basis for immunodominant human T-cell receptor recognition.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	100
Detector technology	CCD
Collection date	2002-08-15
Detector	ADSC CCD
Spacegroup name	P 1 21 1
Unit cell lengths	67.019, 108.838, 77.741
Unit cell angles	90.00, 112.46, 90.00

Refinement procedure

Resolution	30.000 * - 1.400
R-factor	0.218
Rwork	0.218
R-free	0.23200 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1qsf
RMSD bond length	0.008
RMSD bond angle	25.600 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	EPMR
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.450
High resolution limit [Å]	1.400	1.400
Rmerge	0.063	0.830
Total number of observations	3465000 *
Number of reflections	200813
<I/σ(I)>	38.5	1.9
Completeness [%]	99.4	98.3
Redundancy	17.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	6.5		14% PEG8000, 50MM MES PH 6.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	PEG8000	14 (%)
3	1	drop	MES	50 (mM)	pH6.5