1OG6
ydhF, an aldo-keto reductase from E.coli complexed with NADPH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-12-15 |
| Detector | ADSC CCD |
| Spacegroup name | H 3 |
| Unit cell lengths | 174.529, 174.529, 97.923 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.910 - 2.800 |
| R-factor | 0.207 |
| Rwork | 0.207 |
| R-free | 0.23800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.800 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.880 | 2.980 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.105 | 0.381 |
| Number of reflections | 27329 | |
| <I/σ(I)> | 6.1 | 1.8 |
| Completeness [%] | 99.0 | 96 |
| Redundancy | 4.8 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | IMIDAZOLE/MALATE 0.2M AMMONIUM SULFATE 0.1M PH7.0, POLY ETHYLENE GLYCOL 16%, pH 7.00 |
