1OFP
CRYSTAL STRUCTURE OF THE TYROSINE-REGULATED 3-DEOXY-D-ARABINO-HEPTULOSONATE-7-PHOSPHATE SYNTHASE FROM SACCHAROMYCES CEREVISIAE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 105 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-07-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 |
| Unit cell lengths | 51.590, 69.004, 102.450 |
| Unit cell angles | 106.38, 101.48, 94.81 |
Refinement procedure
| Resolution | 19.880 - 2.100 |
| Rwork | 0.205 |
| R-free | 0.26000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hfb |
| RMSD bond length | 0.043 |
| RMSD bond angle | 2.864 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR |
| Refinement software | REFMAC (5.1.19) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.900 | 2.150 |
| High resolution limit [Å] | 2.000 | 2.030 |
| Rmerge | 0.059 | 0.364 |
| Number of reflections | 414373 | |
| <I/σ(I)> | 16.1 | 3.3 |
| Completeness [%] | 97.4 | 91.5 |
| Redundancy | 4.9 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | TRIS PH 7.5-9.0 10 MM 20% PEG3400, 13-17MG/ML DAHPS |
