1OFO
Crystal Structure of the Tyrosine Regulated 3-Deoxy-D-Arabino-Heptulosonate-7-Phosphate Synthase from Saccharomyces Cerevisiae in Complex with 2-Phosphoglycolate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ROTATING ANODE (CU) |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-06-15 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 196.457, 50.955, 64.976 |
| Unit cell angles | 90.00, 106.41, 90.00 |
Refinement procedure
| Resolution | 17.300 - 1.860 |
| Rwork | 0.165 |
| R-free | 0.20900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1HFB (MOLECULE A) |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.728 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR |
| Refinement software | REFMAC (5.1.19) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 17.300 | 1.950 |
| High resolution limit [Å] | 1.860 | 1.860 |
| Rmerge | 0.044 | 0.303 |
| Number of reflections | 198507 | |
| <I/σ(I)> | 19.77 | 4.36 |
| Completeness [%] | 95.4 | 89.1 |
| Redundancy | 3.8 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | pH 8.00 |
